Difference between revisions of "Part:BBa K801077"

Revision as of 22:57, 26 September 2012

Caffeine Synthesis Pathway pTEF2-XMT1-tADH1-pTEF1-MXMT1-tADH1-pTEF2-DXMT1-tADH1

This BioBrick is the final "caffeine synthesis device". It contains all three necessary enzymes: CaXMT1, CaMXMT1 and CaDXMT1, i.e. it is made up of the three single expression cassettes for each enzyme (see also BBa_K801073, BBa_K801074 and [https://parts.igem.org/Part:BBa_K801075 BBa_K801075). It can be directly transformed into competent yeast cells or cloned in an adequate yeast genome integration vector.

The expression of the enzymes CaXMT1 (Coffea arabica xanthosine-N-methyl-transferase 1, BBa_K801070) and CaDXMT1 (Coffea arabica dimethyl-xanthine N-methyl transferase 1, = caffeine synthase, BBa_K801072) is controlled by the TEF2-promoter (BBa_K801010) and the ADH1-terminator (BBa_K801012)

The expression of CaMXMT1 (Coffea arabica methyl-xanthine N-methyl transferase 1, BBa_K801071) is controlled by the TEF1 promoter (BBa_K319003) and the ADH1-terminator (BBa_K801012)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3613
Illegal BglII site found at 5829
Illegal BglII site found at 5925
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 138
Illegal NgoMIV site found at 4317
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2385

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K801077 short</partinfo>
-This part allows the expression of the three enzymes CaXMT1, CaMXMT1 and CaDXMT1 in '''yeast'''. These enzymes form the biosynthesis pathway for caffeine.
+This BioBrick is the final "caffeine synthesis device". It contains all three necessary enzymes:  CaXMT1, CaMXMT1 and CaDXMT1, i.e. it is made up of the three single expression cassettes for each enzyme (see also [https://parts.igem.org/Part:BBa_K801073 BBa_K801073], [https://parts.igem.org/Part:BBa_K801074 BBa_K801074 and [https://parts.igem.org/Part:BBa_K801075 BBa_K801075]). It can be directly transformed into competent yeast cells or cloned in an adequate yeast genome integration vector.
@@ Line 9: / Line 9: @@
 The expression of CaMXMT1 (''Coffea arabica'' methyl-xanthine N-methyl transferase 1, BBa_K801071) is controlled by the TEF1 promoter (BBa_K319003) and the ADH1-terminator (BBa_K801012)
 <!-- Add more about the biology of this part here