Difference between revisions of "Part:BBa K782072"
| Line 35: | Line 35: | ||
==References== | ==References== | ||
| − | Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698 | + | Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698. |
Latest revision as of 22:55, 26 September 2012
12x[TALD] operator
- TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).
- DNA binding sites for individual TAL effectors are indicated with square brackets [ ].
Introduction
TAL effectors (TALEs) are able to specifically bind to the DNA by recognizing base pairs on a DNA molecule. With TAL regulators we are able to control expression of a desired gene. Efficient transcription control can be achieved by binding of multiple TAL regulators upstream of promoter.
Our construct contain twelve specific binding sites for TALD (Figure 1).
Single binding sequence for TALD is: TCGTCCAATAGCTTCTC
Figure 1. Shematic representation of the construct.
- Binding sites for TAL effectors were ordered from IDT.
Characterization
Construct was later used for constructing different reporter plasmids to show specific binding of TALD:KRAB/TALD:VP16.
Results:
References
Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 214
Illegal AgeI site found at 446 - 1000COMPATIBLE WITH RFC[1000]