Difference between revisions of "Part:BBa K782069"

Latest revision as of 22:51, 26 September 2012

10x[TALA+TALB] operator

• TALA and TALB labels represents TAL effector 1257 and 1297 from zebrafish experiments (Sander et al., 2011).
• DNA binding sites for individual TAL effectors are indicated with square brackets [ ].

Introduction

TAL effectors (TALEs) are able to specifically bind to the DNA by recognizing base pairs on a DNA molecule. With TAL regulators we are able to control expression of a desired gene. Efficient transcription control can be achieved by binding of multiple TAL regulators upstream of promoter.

Our construct contain ten binding sites for TALA and another ten for TALB. Single binding site is combined from 18 base pairs recognised by specific TAL. TALA and TALB binding sites are adjacent to each other making impossible for both of them to bind at once.

Single binding site sequence for TALA: TTTACTGCTGCTCCCGCT

Single binding site sequence for TALB: TCTTCCGTTTCCACATCT

Figure 1: Schematic representation of the construct.

• Binding sites for TAL effectors were ordered from GeneArt.

Characterization

Construct was later used for constructing different reporter plasmids to show specific binding and competition between TALA and TALB.

Results:

• BBa_K782028
• BBa_K782019
• BBa_K782016

References

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.

Sequence and Features