Difference between revisions of "Part:BBa K845000"

Revision as of 22:48, 26 September 2012

iGEM 2012 Grenoble Team proposes a new design and application to the pBAD promoter(paraBAD).

In order to create a biological AND gate the 2012 Grenoble team worked on the cAMP-CRP complex and arabinose inductible promoter.

The activity of the promoter was tested under several conditions ( http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test ) using a GFP as a reporter (on a pUA66). We tested this activity in BW25113 ΔcyaA in different culture media (Glucose + Acetate; Glycerol; Acetate).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 500
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 344
Illegal XhoI site found at 494
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 500
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 500
Illegal AgeI site found at 179
Illegal AgeI site found at 484
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1169
Illegal SapI site found at 161

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K845000 short</partinfo>
@@ Line 5: / Line 4: @@
 In order to create a biological AND gate the 2012 Grenoble team worked on the cAMP-CRP complex and arabinose inductible promoter.
-The activity of the promoter was tested under several conditions (http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test)using a GFP as a reporter (on a pUA66).
+The activity of the promoter was tested under several conditions ( http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test ) using a GFP as a reporter (on a pUA66).
 We tested this activity in BW25113 &#916;cyaA in different culture media (Glucose + Acetate; Glycerol; Acetate).