Difference between revisions of "Part:BBa C0012"
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__NOTOC__
 
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<partinfo>BBa_C0012 short</partinfo>
 
<partinfo>BBa_C0012 short</partinfo>
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A rapid degredation tail (LVA) has been added to improve the High to Low      performance of this part.
 
A rapid degredation tail (LVA) has been added to improve the High to Low      performance of this part.
  
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[[Image:LacRep-black.jpg]]
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_C0012 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_C0012 parameters</partinfo>
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Revision as of 02:43, 5 June 2006

lacI repressor from E. coli (+LVA)

Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.

A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.

LacRep-black.jpg