Difference between revisions of "Part:BBa K812331"

(New page: __NOTOC__ <partinfo>BBa_K812331 short</partinfo> <html> </br> Super folded GFP fused with a kozak sequence to enhance its expression in eukaryotic cells. This part is in pCS2+, the plasmi...)
 
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</br>This part was  created with the plasmid backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a> and J04450 was replaced by sfGFP (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812031</a>).  
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</br>This part was  created with the plasmid backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>: J04450 was replaced by sfGFP (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812031</a>) and the CMV promoter was replaced by the Hsp70 promoter promoter with SalI and HindIII restriction sites from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812300</a>.  
  
  

Revision as of 22:36, 26 September 2012

sfGFP in pCS2+ with HSP70 promoter


Super folded GFP fused with a kozak sequence to enhance its expression in eukaryotic cells. This part is in pCS2+, the plasmid backbone is BBa_K812000
This part was created with the plasmid backbone BBa_K812000: J04450 was replaced by sfGFP (BBa_K812031) and the CMV promoter was replaced by the Hsp70 promoter promoter with SalI and HindIII restriction sites from BBa_K812300.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 19