Difference between revisions of "Part:BBa K782067"
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* TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).
 
* TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).
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* NA binding sites for individual TAL effectors are indicated with square brackets [ ].
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* Binding sites for individual TAL effectors are indicated with square brackets [ ].
  
  

Revision as of 22:29, 26 September 2012

2x[NicTAL]+2x[TALD] operator

  • TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).
  • Binding sites for individual TAL effectors are indicated with square brackets [ ].


Introduction

Our construct contains two specific binding sites for NicTAL12 and TALD.

Single binding sequence for NicTAL12 is: TCTATCAATGATAGA

Single binding sequence for TALD is: TCGTCCAATAGCTTCTC


2xNic+2xD.png

Figure 1. Shematic representation of two consecutive specific binding sites for NicTAL12 and TALD.


  • Binding sites for TAL effectors were ordered from GeneArt.


Characterization

Specific TAL binding sites were further characterized with different reporter constructs.

Results:


References

Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 35
    Illegal AgeI site found at 105
  • 1000
    COMPATIBLE WITH RFC[1000]