Difference between revisions of "Part:BBa K818400"

m
Line 6: Line 6:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 +
'''Expression in <i>E. coli</i>'''
 +
SboA-AmilCP is strongly expressed in <i>E. coli</i>, on plate and in liquid culture, at normal growth conditions. On plate, the blue-purple colour is less visible compared to the cell pellet in liquid culture.
  
 +
'''Expression in <i>B. subtilis</i>'''
 +
 +
SboA-AmilGFP was shown to be very weakly expressed in <i>Bacillus subtilis</i> on LB plate without rotten meat induction (faint color formation after 2 days). This is probably due to the leakiness of the promoter.
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 22:01, 26 September 2012

AmilCP with sboA regulator

This is a coding construct for B. subtilis to produce AmilCP pigment in response to rotten meat volatiles in the environment. AmilCP is a blue pigment designed by iGEM Uppsala 2011 and the regulator, sboA, is designed by iGEM Groningen 2012.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]