Difference between revisions of "Part:BBa K738002:Design"

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===Design Notes===
 
===Design Notes===
 
Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (''rne''131) gene to limit RNA degradation.
 
Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (''rne''131) gene to limit RNA degradation.
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===Safety Notes===
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The gene products of K738000 are RNAs and they will not be translated into proteins. As we know, RNA can be easily degraded, so our biobricks will not raise potentially safety problems.
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===Source===
 
===Source===

Revision as of 21:57, 26 September 2012

Theophyline riboswitch regulated RNA Scaffold (clover vision 2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (rne131) gene to limit RNA degradation.

Safety Notes

The gene products of K738000 are RNAs and they will not be translated into proteins. As we know, RNA can be easily degraded, so our biobricks will not raise potentially safety problems.


Source

Clover version 2 was synthesized by Genescript.

References

C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, “Organization of intracellular reactions with rationally designed RNA assemblies” Science 333, 470-474 (2011).