Difference between revisions of "Part:BBa K801012"
(→‎Design Notes)
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<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
 
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
  
−
'''Cloning details:'''<br>
+
'''Cloning details:'''<br> Designed in RFC10/RFC23/RFC25/RFC25
 
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
 
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
  
−
'''Quality control measures:'''<br>
+
'''Quality control measures:'''<br> Test digestion using XbaI+ PstI-HF
 +
*Sequencing using primer VF2 and VR
 
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 
<!--*Part was partly sequenced/Part was totally sequenced-->
 
<!--*Part was partly sequenced/Part was totally sequenced-->
  
−
'''Backbone:'''<br>
+
'''Backbone:'''<br> Backbone name: pSB1C3
 +
* Resistance: Cp
 
<!--*Backbone name: pSB1C3'/?backbone_name?-->
 
<!--*Backbone name: pSB1C3'/?backbone_name?-->
 
<!--*Resistance: Amp/Cp/Kan/Tet-->
 
<!--*Resistance: Amp/Cp/Kan/Tet-->
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<!--none/not known/cytotoxic for ''organism name''-->
 
<!--none/not known/cytotoxic for ''organism name''-->
  
−
'''Safety notes:'''<br>
+
'''Safety notes:'''<br> none
 
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 
<!--Known and anticipated security issues: none/other.-->
 
<!--Known and anticipated security issues: none/other.-->
  
−
'''Intellectual property:'''
+
'''Intellectual property:''' PCR-Product from Vector pGADT7 AD from Clontech
−
<!--Information on patent situation.-->
+
<!--Information on patent situation.-->  
 
<!--Intellectual property claims made by the authors.-->
 
<!--Intellectual property claims made by the authors.-->
  

Revision as of 21:39, 26 September 2012

Terminator.png

ADH1 yeast terminator

  • ADH1 terminator of S. cerevisiae

Secondary Structure

File:Mfold-K801012-1.png

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]



Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:
Test digestion using XbaI+ PstI-HF

  • Sequencing using primer VF2 and VR

Backbone:
Backbone name: pSB1C3

  • Resistance: Cp

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:
none

Intellectual property: PCR-Product from Vector pGADT7 AD from Clontech

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: