Difference between revisions of "Part:BBa K864013"

Revision as of 21:22, 26 September 2012

Low copy BioBrick standard vector with LacI

pSB4C15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin BBa_K864050 (~5 copies per cell) and chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.

This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.

The resistance cassette is flanked by FRT sites (BBa_J61020) which allows for subsequent removal of the resistance cassette from the chromosome by the FLP recombinase.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4693
Illegal NheI site found at 2425
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4699
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4693
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 4693
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 4693
Plasmid lacks a suffix.
Illegal XbaI site found at 4708
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 2319
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K864013 short</partinfo>
-pSB4C15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
+pSB4C15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin <partinfo>BBa_K864050</partinfo> (~5 copies per cell) and chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
 This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.