Difference between revisions of "Part:BBa K873003"
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easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation | easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation | ||
rate, conjugation time, and conjugation efficiency between different strains. | rate, conjugation time, and conjugation efficiency between different strains. | ||
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== Results == | == Results == | ||
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[[Image:seulfig5.jpg]] [[Image:seulfig4.jpg]] | [[Image:seulfig5.jpg]] [[Image:seulfig4.jpg]] | ||
| − | It's show that the conjugation system did work. For more information on how this part operates in our Detection system, please visit | + | |
| + | It's show that the conjugation system did work. | ||
| + | For more information on how this part operates in our Detection system, please visit [http://2012.igem.org/Team:SEU_A SEU_A ]． | ||
== references == | == references == | ||
Revision as of 20:48, 26 September 2012
conjugative transfer of RFG
This part contains a reporter gene I13521,combined with OriTR(the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.)
Function
Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation rate, conjugation time, and conjugation efficiency between different strains.
Results
The TEM image of conjugation:
The agar Luria-Bertani medium of DH5α: -------------------------------------------------------------- The agar Luria-Bertani medium of BL21:
The agar Luria-Bertani medium of HB101: -------------------------------------------------------------- The agar Luria-Bertani medium of conjugation:
It's show that the conjugation system did work. For more information on how this part operates in our Detection system, please visit [http://2012.igem.org/Team:SEU_A SEU_A ]．
references
[http://onlinelibrary.wiley.com/doi/10.1002/bmb.113/full]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 117
Illegal NgoMIV site found at 127
Illegal AgeI site found at 1014
Illegal AgeI site found at 1126 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 19