Difference between revisions of "Part:BBa K812133"

Revision as of 20:47, 26 September 2012

sfGFP with kozak sequence for eukaryotic expression

Super folded GFP fused with a kozak sequence to enhance its expression in eukaryotic cells. This part is in pCS2+, the plasmid backbone is K812000
This part was created with the plasmid backbone K812000 and J04450 was replaced by sfGFP (K812031). This plasmid express sfGFP into Xenopus:
perdu

The expression of sfGFP is present only in one of the two embryo/tadpole. sfGFP is present only in few tissue as others reporters. sfGFP is expressed in tail's muscles and branchial basket for this tadpole. After injection of this plasmid into Xenopus it does not diffuse in the eggs, and the plasmid express sfGFP in few different tissues.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 19

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 <html>
+</br>
 Super folded GFP fused with a kozak sequence to enhance its expression in eukaryotic cells. This part is in pCS2+, the plasmid backbone is <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">K812000</a>
-</br><br/>This part was  created with the plasmid backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">K812000</a>  and J04450 was replaced by sfGFP (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">K812031</a>). This plasmid express sfGFP into Xenopus: <br/>
+</br>This part was  created with the plasmid backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">K812000</a>  and J04450 was replaced by sfGFP (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">K812031</a>). This plasmid express sfGFP into Xenopus: <br/>
 <img src="https://static.igem.org/mediawiki/2012/2/27/PCS2%2B_sfGFP_20%2621_09.png" alt="perdu" width="880px" /> <br/><br/>