Difference between revisions of "Part:BBa K778011"

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__NOTOC__
 
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<partinfo>BBa_K778011 short</partinfo>
 
<partinfo>BBa_K778011 short</partinfo>
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This device allows you to "enhance" and "see" H2 production. 
  
 
hycAp-GFPgenerator-plac-FhlA363generator
 
hycAp-GFPgenerator-plac-FhlA363generator

Revision as of 20:32, 26 September 2012

hycAp-GFPgenerator-plac-FhlA363generator

This device allows you to "enhance" and "see" H2 production.

hycAp-GFPgenerator-plac-FhlA363generator

GFP is a reportor of H2 production (strictly speaking,reporter of hycAp activation Part:BBa K778000 (hycAp is the promotor of formate hydrogen lyase of genome)).

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). fhlA-E363G has one point mutation (A to G, glutamic acid to glycine). Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2545
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 828