Difference between revisions of "Part:BBa K896002"

Revision as of 20:17, 26 September 2012

Dsr (sulfite reductase)

Our engingeered cyanobacteria can remove Sulfur Oxides (SOX, SO2),main precursors of air pollution and produce H2S for futher uses(Sulfide-Quinone Reductase,sqr).

[http://www.rcsb.org/pdb/explore/explore.do?structureId=3OR1 Crystal structure of dissimilatory sulfite reductase I (DsrI)]

[http://www.rcsb.org/pdb/explore/explore.do?structureId=3OR2 Crystal structure of dissimilatory sulfite reductase II (DsrII)]

Usage and Biology

1. By paper surveying, we found out a kind of anaerobe bacteria, sulfur reducing bacteria(SRB), which have powerful ability to reduce (HSO3)- to H2S.

2. We discussed eith several specialists and searched for KEGG and NCBI. Finally we engingeered a common SRB, Desulfovibrio desulfuricans ATCC27774 and cloned its sulfite reductase, Dsr.

3.There are two sulfite reductase operon, DsrI and DsrII, inside Desulfovibrio desulfuricans. They might be membrane form Dsr operon and free form Dsr operon.

4. We've already constructed Dsr gene into E.coli and cyanobacteria and used bacteria to remove SO2 in our environment. Also, this bacteria produced H2S, which is a substrate for Sulfide-Quinone Reductase(sqr).

5.These potential Dsr transformed bacteria can perform bioremediation, also are a powerful species for Veniusian!!

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1636
Illegal EcoRI site found at 4648
Illegal PstI site found at 826
Illegal PstI site found at 3838
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1636
Illegal EcoRI site found at 4648
Illegal PstI site found at 826
Illegal PstI site found at 3838
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1636
Illegal EcoRI site found at 4648
Illegal BglII site found at 1091
Illegal BglII site found at 4103
Illegal BamHI site found at 3007
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1636
Illegal EcoRI site found at 4648
Illegal PstI site found at 826
Illegal PstI site found at 3838
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1636
Illegal EcoRI site found at 4648
Illegal PstI site found at 826
Illegal PstI site found at 3838
Illegal NgoMIV site found at 1741
Illegal NgoMIV site found at 4753
Illegal AgeI site found at 406
Illegal AgeI site found at 1261
Illegal AgeI site found at 3418
Illegal AgeI site found at 4273
1000
COMPATIBLE WITH RFC[1000]

Details are in Design Notes!!

Functional Parameters

kegg	Desulfovibrio desulfuricans ATCC 27774

@@ Line 13: / Line 13: @@
 '''[http://www.rcsb.org/pdb/explore/explore.do?structureId=3OR2 Crystal structure of dissimilatory sulfite reductase II (DsrII)]'''
+===Usage and Biology===
+. By paper surveying, we found out a kind of anaerobe bacteria, sulfur reducing bacteria(SRB), which have powerful ability to reduce (HSO3)- to H2S.
+. We discussed eith several specialists and searched for KEGG and NCBI. Finally we engingeered a common SRB, Desulfovibrio desulfuricans ATCC27774 and cloned its sulfite reductase, Dsr.
+.There are two sulfite reductase operon, DsrI and DsrII, inside Desulfovibrio desulfuricans. They might be membrane form Dsr operon and free form Dsr operon.
+. We've already constructed Dsr gene into E.coli and cyanobacteria and used bacteria to remove SO2 in our environment. Also, this bacteria produced H2S, which is a substrate for Sulfide-Quinone Reductase(sqr).
+.These potential Dsr transformed bacteria can perform bioremediation, also are a powerful species for Veniusian!!
@@ Line 19: / Line 33: @@
-===Usage and Biology===
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K896002 SequenceAndFeatures</partinfo>
 Details are in [https://parts.igem.org/Part:BBa_K896002:Design Design Notes]!!
-<!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K896002 parameters</partinfo>
-<!-- -->