Difference between revisions of "Part:BBa K778002"

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<partinfo>BBa_K778002 short</partinfo>
 
<partinfo>BBa_K778002 short</partinfo>
  
FhlA is the trnascriptional activator of hycAp (Part:BBa_778000). E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
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FhlA is the trnascriptional activator of hycAp [[Part:BBa K778000]] .''E.coli'' overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
  
 
fhlA-E363G has one point mutation (A to G, glutamic acid to glycine).
 
fhlA-E363G has one point mutation (A to G, glutamic acid to glycine).
 
  Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]
 
  Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 19:52, 26 September 2012

fhlA-E363G (engineered trascriptional activator fhlA with a mutation)

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 .E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).

fhlA-E363G has one point mutation (A to G, glutamic acid to glycine).

Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2096
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1375
  • 1000
    COMPATIBLE WITH RFC[1000]