Difference between revisions of "Part:BBa K863120:Design"
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<partinfo>BBa_K863120 short</partinfo> | <partinfo>BBa_K863120 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | Added Freiburg-restriction-sites for in frame assembly. | + | Added Freiburg-restriction-sites for in frame assembly. |
| − | + | ||
| + | Primer that were used to made this BioBrick: | ||
| + | {| | ||
| + | |GFP_Freiburg||ACGTGAATTCGCGGCCGCTTCTAGATGGCCGGCCGTAAAGGAGAAGAACTTTTC | ||
| + | |- | ||
| + | |GFP_Freiburg_compl||ACGTCTGCAGCGGCCGCTACTAGTATTAACCGGTTTTGTATAGTTCATCCATGCCATGTGT | ||
| + | |} | ||
===Source=== | ===Source=== | ||
Revision as of 19:47, 26 September 2012
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Freiburg-Standard)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 721 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 650
Design Notes
Added Freiburg-restriction-sites for in frame assembly.
Primer that were used to made this BioBrick:
| GFP_Freiburg | ACGTGAATTCGCGGCCGCTTCTAGATGGCCGGCCGTAAAGGAGAAGAACTTTTC |
| GFP_Freiburg_compl | ACGTCTGCAGCGGCCGCTACTAGTATTAACCGGTTTTGTATAGTTCATCCATGCCATGTGT |