Difference between revisions of "Part:BBa K863121:Design"
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===Design Notes=== | ===Design Notes=== | ||
Added Freiburg-restriction-sites for in frame assembly and a His-tag. | Added Freiburg-restriction-sites for in frame assembly and a His-tag. | ||
+ | |||
+ | Primers that were used to make this BioBrick: | ||
+ | {| | ||
+ | |GFP_Frei||TACGGAATTCGCGGCCGCTTCTAGATGGCCGGCATGCGTAAAGGAGAAGAACTT | ||
+ | |- | ||
+ | |GFP_His6_compl||CTGCAGCGGCCGCTACTAGTATTAACCGGTGTGATGGTGATGGTGATGTTTGTATAGTTCATCCATGCCATGTG | ||
+ | |} | ||
===Source=== | ===Source=== |
Revision as of 19:42, 26 September 2012
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (His-tag)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 742 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 653
Design Notes
Added Freiburg-restriction-sites for in frame assembly and a His-tag.
Primers that were used to make this BioBrick:
GFP_Frei | TACGGAATTCGCGGCCGCTTCTAGATGGCCGGCATGCGTAAAGGAGAAGAACTT |
GFP_His6_compl | CTGCAGCGGCCGCTACTAGTATTAACCGGTGTGATGGTGATGGTGATGTTTGTATAGTTCATCCATGCCATGTG |