Difference between revisions of "Part:BBa K861171:Experience"

(→‎Applications of BBa_K861171)
(→‎Applications of BBa_K861171)
Line 5: Line 5:
 
===Applications of BBa_K861171===
 
===Applications of BBa_K861171===
 
<html>
 
<html>
−
<h>Methods</h>
+
<h2>Methods</h2>
−
<h2>Design of the promoter Pcar which is activated by glucose </h2>
+
<3>Design of the promoter Pcar which is activated by glucose </h3>
 
<p>
 
<p>
 
   Promoter Pcar , glucose biosensor plasmid, is derived from constitutive promoter (BBa_J23119) by adding a CRP binding site upstream the promoter which has several base pairs overlapping with polymerase binding site. The sequence was synthesized with restriction enzyme cutting site for EcoRI and XbaI at the 5' terminal and SpeI at 3' terminal. The sequence of promoter Pcar has cohesive terminus at both ends, so it is very convenient for us to construct the plasmid for functional detection.The sequence of Pcar is as followed:
 
   Promoter Pcar , glucose biosensor plasmid, is derived from constitutive promoter (BBa_J23119) by adding a CRP binding site upstream the promoter which has several base pairs overlapping with polymerase binding site. The sequence was synthesized with restriction enzyme cutting site for EcoRI and XbaI at the 5' terminal and SpeI at 3' terminal. The sequence of promoter Pcar has cohesive terminus at both ends, so it is very convenient for us to construct the plasmid for functional detection.The sequence of Pcar is as followed:

Revision as of 19:41, 26 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K861171

Methods

<3>Design of the promoter Pcar which is activated by glucose

Promoter Pcar , glucose biosensor plasmid, is derived from constitutive promoter (BBa_J23119) by adding a CRP binding site upstream the promoter which has several base pairs overlapping with polymerase binding site. The sequence was synthesized with restriction enzyme cutting site for EcoRI and XbaI at the 5' terminal and SpeI at 3' terminal. The sequence of promoter Pcar has cohesive terminus at both ends, so it is very convenient for us to construct the plasmid for functional detection.The sequence of Pcar is as followed:

User Reviews

UNIQ4baa8a0cc3a7e44f-partinfo-00000001-QINU UNIQ4baa8a0cc3a7e44f-partinfo-00000002-QINU