Difference between revisions of "Part:BBa K861171:Experience"
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===Applications of BBa_K861171=== | ===Applications of BBa_K861171=== | ||
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| − | Methods | + | <h>Methods</h> |
| − | Design of the promoter Pcar which is activated by glucose | + | <h2>Design of the promoter Pcar which is activated by glucose </h2> |
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Promoter Pcar , glucose biosensor plasmid, is derived from constitutive promoter (BBa_J23119) by adding a CRP binding site upstream the promoter which has several base pairs overlapping with polymerase binding site. The sequence was synthesized with restriction enzyme cutting site for EcoRI and XbaI at the 5' terminal and SpeI at 3' terminal. The sequence of promoter Pcar has cohesive terminus at both ends, so it is very convenient for us to construct the plasmid for functional detection.The sequence of Pcar is as followed: | Promoter Pcar , glucose biosensor plasmid, is derived from constitutive promoter (BBa_J23119) by adding a CRP binding site upstream the promoter which has several base pairs overlapping with polymerase binding site. The sequence was synthesized with restriction enzyme cutting site for EcoRI and XbaI at the 5' terminal and SpeI at 3' terminal. The sequence of promoter Pcar has cohesive terminus at both ends, so it is very convenient for us to construct the plasmid for functional detection.The sequence of Pcar is as followed: | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 19:41, 26 September 2012
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Applications of BBa_K861171
Design of the promoter Pcar which is activated by glucose
Promoter Pcar , glucose biosensor plasmid, is derived from constitutive promoter (BBa_J23119) by adding a CRP binding site upstream the promoter which has several base pairs overlapping with polymerase binding site. The sequence was synthesized with restriction enzyme cutting site for EcoRI and XbaI at the 5' terminal and SpeI at 3' terminal. The sequence of promoter Pcar has cohesive terminus at both ends, so it is very convenient for us to construct the plasmid for functional detection.The sequence of Pcar is as followed:
User Reviews
UNIQ8784ded034d3a8c1-partinfo-00000001-QINU UNIQ8784ded034d3a8c1-partinfo-00000002-QINU