Difference between revisions of "Part:BBa K778002"
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fhlA-E363G has one point mutation (A to G, glutamic acid to glycine). | fhlA-E363G has one point mutation (A to G, glutamic acid to glycine). | ||
| − | Detailed Information :[ | + | Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 19:21, 26 September 2012
fhlA-E363G (engineered trascriptional activator fhlA with a mutation)
FhlA is the trnascriptional activator of hycAp (Part:BBa_778000). E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
fhlA-E363G has one point mutation (A to G, glutamic acid to glycine).
Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2096
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1375
- 1000COMPATIBLE WITH RFC[1000]