Difference between revisions of "Part:BBa K819010"

Revision as of 19:11, 26 September 2012

Medium cheZ generator

CheZ is a significant protein in E.coli chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.
CheZ was placed under a weak promoter BBa_J23113. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain.

Figure 1. E.coli was grown on semi-solid medium. (a) MG1655 strain with cheZ gene deleted; (b) CheZ gene under weak promoter BBa_J23112 (BBa_K819009); (c) CheZ gene under medium promoter BBa_J23113 (this part); (d)wild type MG1655 strain;

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <img src="https://static.igem.org/mediawiki/parts/c/c2/Peking2012_part_CheZ_K819010.png" style="width:500px;margin-left:10%;"/>
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-<p>Figure 1. <i>E.coli</i> was grown on semi-solid medium. a)MG1655 strain with cheZ gene deleted. b)cheZ gene under weak promoter BBa_J23112 ([https://parts.igem.org/Part:BBa_K819009 BBa_K819009]). <b>c)cheZ gene under medium promoter BBa_J23113 (this part)</b> d)wild type MG1655 strain</p>
+<p>Figure 1. <i>E.coli</i> was grown on semi-solid medium. (a) MG1655 strain with cheZ gene deleted; (b) CheZ gene under weak promoter BBa_J23112 ([https://parts.igem.org/Part:BBa_K819009 BBa_K819009]); (c) CheZ gene under medium promoter BBa_J23113 (this part); (d)wild type MG1655 strain;</p>
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