Difference between revisions of "Part:BBa K733010"

Revision as of 17:39, 26 September 2012

Ptms+RBS+ydcD.

This construct contains a low efficient constitutive promoter Ptms with an antitoxin gene ydcD (endB). The gene ydcD can express a protein named YdcD, which can counteract with a protein EndoA expressed by a toxin gene ydcE (ndoA). (Pellegrini et al., 2005) Besides, the low efficiency promoter will contribute to the low level expression of YdcD.

Ptms promoter: BBa_K733001

ydcD: BBa_K733003

ydcE: BBa_K733004

Cell Growth Inhibition Device: BBa_K733012

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Reference

Pellegrini O, Mathy N, Gogos A, Shapiro L, and Condon C. "The Bacillus subtilis' ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.." Molecular microbiology. 56.5 (2005): 1139-1148. Print.

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K733010 short</partinfo>
-This construct contains a low efficient constitutive promoter pTms with an antitoxin gene ''ydcD (endB)''. The gene ''ydcD'' can express a protein named YdcD, which can counteract with a protein EndoA expressed by a toxin gene ''ydcE (ndoA)''.   (Pellegrini et al., 2005) Besides, the low efficiency promoter will contribute to the low level expression of YdcD.
+This construct contains a low efficient constitutive promoter ''Ptms'' with an antitoxin gene ''ydcD (endB)''. The gene ''ydcD'' can express a protein named YdcD, which can counteract with a protein EndoA expressed by a toxin gene ''ydcE (ndoA)''.   (Pellegrini et al., 2005) Besides, the low efficiency promoter will contribute to the low level expression of YdcD.
 ''Ptms'' promoter: [https://parts.igem.org/Part:BBa_K733001 BBa_K733001]