Difference between revisions of "Part:BBa I742111:Experience"

Revision as of 17:38, 26 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I742111

A composite LIMS1 part with a Lac promoter has been characterized. For details, please visit this page.

Team TU Munich 2012 used this part as PCR-template to obtain the coding sequence of (+)-Limonene Synthase. See the page of our BioBrick BBa_K801060.

User Reviews

UNIQ9a64f0039e14a43b-partinfo-00000002-QINU

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iGEM Dundee 2011

We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as BBa_K562015). This will allow better monitoring of production of the protein, and it was detectable using an anti-HA monoclonal antibody whenexpressed in E. coli. We did not get the chance to test limonene production in a host cell producing this part.

UNIQ9a64f0039e14a43b-partinfo-00000005-QINU

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 A composite LIMS1 part with a Lac promoter has been characterized. For details, please visit this <html><a href="https://parts.igem.org/Part:BBa_K118025:Experience">page</a>.</html>
-Team TU Munich 2012 used this part as PCR-template to obtain the coding sequence of (+)-Limonene Synthase. See the page of our <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K801060">BioBrick</a>.</html>
+Team TU Munich 2012 used this part as PCR-template to obtain the coding sequence of (+)-Limonene Synthase. See the page of our BioBrick <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K801060">BBa_K801060</a>.</html>
 ===User Reviews===