Difference between revisions of "Part:BBa I742111:Experience"
Simon Heinze (Talk | contribs) (→Applications of BBa_I742111) |
Simon Heinze (Talk | contribs) (→Applications of BBa_I742111) |
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A composite LIMS1 part with a Lac promoter has been characterized. For details, please visit this <html><a href="https://parts.igem.org/Part:BBa_K118025:Experience">page</a>.</html> | A composite LIMS1 part with a Lac promoter has been characterized. For details, please visit this <html><a href="https://parts.igem.org/Part:BBa_K118025:Experience">page</a>.</html> | ||
| − | Team TU Munich 2012 used this part as PCR-template to obtain the coding sequence of (+)-Limonene Synthase. See <html><a href="https://parts.igem.org/Part:BBa_K801060"> | + | Team TU Munich 2012 used this part as PCR-template to obtain the coding sequence of (+)-Limonene Synthase. See the page of our <html><a href="https://parts.igem.org/Part:BBa_K801060">BioBrick</a>.</html> |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 17:23, 26 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I742111
A composite LIMS1 part with a Lac promoter has been characterized. For details, please visit this page.
Team TU Munich 2012 used this part as PCR-template to obtain the coding sequence of (+)-Limonene Synthase. See the page of our BioBrick.
User Reviews
UNIQ4061782ac27e29ec-partinfo-00000002-QINU
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iGEM Dundee 2011 |
We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as BBa_K562015). This will allow better monitoring of production of the protein, and it was detectable using an anti-HA monoclonal antibody whenexpressed in E. coli. We did not get the chance to test limonene production in a host cell producing this part. |
UNIQ4061782ac27e29ec-partinfo-00000005-QINU