Difference between revisions of "Part:BBa K817007:Design"

(→‎Design Notes)
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===Source===
 
===Source===
  
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constitutive promoter-RBS-fadR
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The FadR gene is cloned from ''Escherichia coli'' K12 DH5α genomic DNA. The lac promoter and RBS were from BBa_J04500.
  
 
===References===
 
===References===

Revision as of 17:03, 26 September 2012

plac-RBS-FadR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the functional testing part we designed to test both the FadR and pfad construct. The FadR is from BBa_K817001. This part included a IPTG-inducible Lac promoter and RBS with FadR gene. FadR protein is a repressor for pfad promoter. When it is expressed and there is no enough fatty acid nearby, this FadR protein will shut down pfad promoter's function.

This part had also been sequenced and checked.

Source

The FadR gene is cloned from Escherichia coli K12 DH5α genomic DNA. The lac promoter and RBS were from BBa_J04500.

References