Difference between revisions of "Part:BBa K733011:Design"
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===Source=== | ===Source=== | ||
| − | We digest our xylR+PxylA and ydcE and ligate them. | + | We digest our ''xylR''+''PxylA'' and ''ydcE'' and ligate them. |
| − | Backbone: xylR+PxylA in pSB1C3. Enzyme used: SpeI and PstI-HF. | + | Backbone: ''xylR''+''PxylA'' in pSB1C3. Enzyme used: SpeI and PstI-HF. |
| − | Insert: ydcE. Enzyme used: XbaI and PstI-HF. | + | Insert: ''ydcE''. Enzyme used: XbaI and PstI-HF. |
===References=== | ===References=== | ||
Latest revision as of 16:36, 26 September 2012
xylR+PxylA+RBS+ydcE.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 847
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None.
Source
We digest our xylR+PxylA and ydcE and ligate them.
Backbone: xylR+PxylA in pSB1C3. Enzyme used: SpeI and PstI-HF.
Insert: ydcE. Enzyme used: XbaI and PstI-HF.