Difference between revisions of "Part:BBa K733010:Design"
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===Source=== | ===Source=== | ||
| − | We digest | + | We digest ''Ptms'' and RBS+''ydcD'' and ligate them. |
| − | Backbone: | + | Backbone: ''Ptms'' in pSB1C3. Enzyme used: SpeI and PstI-HF. |
| − | Insert: RBS+ydcD. Enzyme used: XbaI and PstI-HF. Purified by gel purification. | + | Insert: RBS+''ydcD''. Enzyme used: XbaI and PstI-HF. Purified by gel purification. |
===References=== | ===References=== | ||
Latest revision as of 16:34, 26 September 2012
Ptms+RBS+ydcD.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
We digest Ptms and RBS+ydcD and ligate them.
Backbone: Ptms in pSB1C3. Enzyme used: SpeI and PstI-HF.
Insert: RBS+ydcD. Enzyme used: XbaI and PstI-HF. Purified by gel purification.