Difference between revisions of "Part:BBa S05054"
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A part of light sensor, including the PcyA coding sequence(I15009), the OmpR-controlled promoter(ROO82) and the mRFP reporter（I13507）. | A part of light sensor, including the PcyA coding sequence(I15009), the OmpR-controlled promoter(ROO82) and the mRFP reporter（I13507）. | ||
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| + | BBa_S05054 is an intermediate constructed by the conjunction of K081024 and I13507. As a part of light sensor, it contains the PcyA coding sequence(I15009), the OmpR-controlled promoter(ROO82) and the mRFP reporter（I13507. With a combination of J13002 and I15008,this part can produce the enzymes, whicn can catalyzed the two procedures required by conversion of haem into PCB,and offer the OmpC promoter. | ||
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Latest revision as of 16:34, 26 September 2012
K081024:I13507
Construction intermediate
A part of light sensor, including the PcyA coding sequence(I15009), the OmpR-controlled promoter(ROO82) and the mRFP reporter（I13507）.
BBa_S05054 is an intermediate constructed by the conjunction of K081024 and I13507. As a part of light sensor, it contains the PcyA coding sequence(I15009), the OmpR-controlled promoter(ROO82) and the mRFP reporter（I13507. With a combination of J13002 and I15008,this part can produce the enzymes, whicn can catalyzed the two procedures required by conversion of haem into PCB,and offer the OmpC promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 790
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 343
Illegal AgeI site found at 1540
Illegal AgeI site found at 1652 - 1000COMPATIBLE WITH RFC[1000]