Difference between revisions of "Part:BBa K802009:Design"

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===Design Notes===
 
===Design Notes===
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The part has 3 genes (sfp, abrB and lacI) under the control of the xylose inducible Pxyl promoter.
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The part has 3 genes (<i>sfp</i>, <i>abrB</i> and <i>lacI</i>) under the control of the xylose inducible P<sub>xyl</sub> promoter.
  
  
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===References===
 
===References===
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1. Fernandes, P.A.V. et al., Antimicrobial activity of surfactants produced by Bacillus subtilis R14 against multidrug-resistant bacteria, Braz. J. Microbiol. vol.38 no.4 São Paulo Oct./Dec. 2007; <br>
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1. Fernandes, P.A.V. et al., Antimicrobial activity of surfactants produced by <i>Bacillus subtilis</i> R14 against multidrug-resistant bacteria, Braz. J. Microbiol. vol.38 no.4 São Paulo Oct./Dec. 2007; <br>
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2. Al-Ajlani, M.M. et al., Production of surfactin from Bacillus subtilis MZ-7 grown on
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2. Al-Ajlani, M.M. et al., Production of surfactin from <i>Bacillus subtilis</i> MZ-7 grown on pharmamedia commercial medium (2007);<br>
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pharmamedia commercial medium (2007);<br>
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3. Abushadi, H.M. et al., Molecular Characterization of <i>Bacillus subtilis</i> Surfactin Producing Strain and the Factors Affecting its Production, Int. J. Agri. Biol., Vol. 7, No. 3, 2005
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3. Abushadi, H.M. et al., Molecular Characterization of Bacillus subtilis Surfactin
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−
Producing Strain and the Factors Affecting its Production, Int. J. Agri. Biol., Vol. 7, No. 3, 2005
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Revision as of 16:21, 26 September 2012

Sufactin generator and biofilm repressor for B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 760
    Illegal SapI.rc site found at 770


Design Notes

The part has 3 genes (sfp, abrB and lacI) under the control of the xylose inducible Pxyl promoter.


Source

The part was constructed from the assembly of 3 biobricks: BBa_K802006,BBa_K802007 and BBa_K802008.

References

1. Fernandes, P.A.V. et al., Antimicrobial activity of surfactants produced by Bacillus subtilis R14 against multidrug-resistant bacteria, Braz. J. Microbiol. vol.38 no.4 São Paulo Oct./Dec. 2007;
2. Al-Ajlani, M.M. et al., Production of surfactin from Bacillus subtilis MZ-7 grown on pharmamedia commercial medium (2007);
3. Abushadi, H.M. et al., Molecular Characterization of Bacillus subtilis Surfactin Producing Strain and the Factors Affecting its Production, Int. J. Agri. Biol., Vol. 7, No. 3, 2005