Difference between revisions of "Part:BBa K909007"
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<partinfo>BBa_K909007 short</partinfo>  
 
<partinfo>BBa_K909007 short</partinfo>  
  
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[[Image:TetR, tetR-DBD, tetR-DBD-UVR8 represion.jpg|frameless|400px|right|thumb|Figure 1
 
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Truncated version of tetracycline repressor TetR, consisting of 1 – 127 amino acids, containing DNA binding domain. The rest of the protein, alpha 8-10 helixes responsible for tetR dimerization, were removed in order to keep tetR-DBD in monomer form and prevent from efficient DNA binding and transcription repression.  
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Truncated version of tetracycline repressor TetR, consisting of 1 – 127 amino acids, containing DNA binding domain. The rest of the protein, alpha 8-10 helixes responsible for tetR dimerization, were removed in order to keep tetR-DBD in monomer form and prevent from efficient DNA binding and transcription repression (see figure).
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Fusions of TetR-DBD with a dimerizing protein(e.g. UVR8 (link))restores TetR-DBD DNA binding.  
  
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[[Image:TetR, tetR-DBD, tetR-DBD-UVR8 represion.jpg|frameless|upright=3|center|thumb|GFP repression (reporter) by full length TetR, TetR-DBD and dimerizing TetR-DBD-UVR8 fusion
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Revision as of 16:14, 26 September 2012

TetR DNA binding domain containing BamHI site for protein fusions


Truncated version of tetracycline repressor TetR, consisting of 1 – 127 amino acids, containing DNA binding domain. The rest of the protein, alpha 8-10 helixes responsible for tetR dimerization, were removed in order to keep tetR-DBD in monomer form and prevent from efficient DNA binding and transcription repression (see figure). Fusions of TetR-DBD with a dimerizing protein(e.g. UVR8 (link))restores TetR-DBD DNA binding.


GFP repression (reporter) by full length TetR, TetR-DBD and dimerizing TetR-DBD-UVR8 fusion

Usage and Biology

We introduced BamHI site at C terminus for an easy in tetR-DBD frame fusions with other proteins. Thus, it can be used in two hybrid systems for both, homo and hetero dimerizations, also one can use these fusions to turn protein-protein interaction into the repression of transcription.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 382
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]