Difference between revisions of "Part:BBa K738004:Design"

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(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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This part should be expressed in e.coli BL21(DE3) because of its T7 promoter and terminator.
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MS2 is a dimer.
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===Source===
 
===Source===

Latest revision as of 15:23, 26 September 2012

FA-2X-MS2: Split GFP N-terminal domain fused with MS2 protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 835
    Illegal BsaI site found at 1219


Design Notes

MS2 is a dimer.

Source

Dr. Camille J. Delebecque offered us plasmids with MS2 secquence.

References