Difference between revisions of "Part:BBa K802005"
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The linker was cloned into the shuttle vectors BBa_K802004 and BBa_K802003 so that the plasmids would correspond to the iGEM format. | The linker was cloned into the shuttle vectors BBa_K802004 and BBa_K802003 so that the plasmids would correspond to the iGEM format. | ||
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| + | <src img="https://static.igem.org/mediawiki/parts/d/d2/Polylinker.jpg"> | ||
===Construction=== | ===Construction=== | ||
Revision as of 14:52, 26 September 2012
iGEM linker for shuttle vectors BBa_K802003 and BBa_K802004
The linker was cloned into the shuttle vectors BBa_K802004 and BBa_K802003 so that the plasmids would correspond to the iGEM format.
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Construction
This part was constructed by hybridization of two complementary primers. The obtained polylinker has compatible sticky ends with the enzyme restriction sites EcoRI and HindIII. For more information, see Part Design.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]