Difference between revisions of "Part:BBa K873003"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K873003 short</partinfo> | <partinfo>BBa_K873003 short</partinfo> | ||
− | + | This part contains a reporter gene I13521,combined with OriTR(the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.) | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency easily though observation. In addition, it facilitates the Follow-up experiments on the conjugation rate, conjugation time, and conjugation efficiency between different strains. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 14:42, 26 September 2012
conjugative transfer of RFG This part contains a reporter gene I13521,combined with OriTR(the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.) Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 117
Illegal NgoMIV site found at 127
Illegal AgeI site found at 1014
Illegal AgeI site found at 1126 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 19