Difference between revisions of "Part:BBa K805013:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | sequence confirmed | |
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===Source=== | ===Source=== | ||
Latest revision as of 14:42, 26 September 2012
CcdB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 216
Design Notes
sequence confirmed
Source
Extracted from the plasimd pEntr-3C
References
Hu LL, Zhang SS, Li XX, Wang BL.The use of the ccdB lethal gene for constructing a zero background vector in order to clone blunt-end PCR products.Mol Biol (Mosk). 2010 Jan-Feb;44(1):174-6.