Difference between revisions of "Part:BBa K802005"
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===Construction===
 
===Construction===
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This part was constructed by the hybridization of two complementary primers. The obtained polylinker has compatible sticky ends with the enzyme restriction sites EcoRI and HindIII. For more information, see Part Design.
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This part was constructed by hybridization of two complementary primers. The obtained polylinker has compatible sticky ends with the enzyme restriction sites EcoRI and HindIII. For more information, see Part Design.
  
 
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Revision as of 14:38, 26 September 2012

iGEM linker for shuttle vectors BBa_K802003 and BBa_K802004

The linker was cloned into the shuttle vectors BBa_K802004 and BBa_K802003 so that the plasmids would correspond to the iGEM format.


Construction

This part was constructed by hybridization of two complementary primers. The obtained polylinker has compatible sticky ends with the enzyme restriction sites EcoRI and HindIII. For more information, see Part Design.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]