Difference between revisions of "Part:BBa K883402"
| Line 4: | Line 4: | ||
This part encodes the major coat protein of the Potato Leafroll Virus (PLRV). It was isolated from a PLRV infected Bildstar potato leaf by iGEM team Wageningen UR 2012. This was done by cDNA synthesis followed by PCR. | This part encodes the major coat protein of the Potato Leafroll Virus (PLRV). It was isolated from a PLRV infected Bildstar potato leaf by iGEM team Wageningen UR 2012. This was done by cDNA synthesis followed by PCR. | ||
| − | The PLRV major Coat Protein should be capable of forming a Virus-Like Particle, but this was not (yet) confirmed for this part. Expression of a similar viral coat proteins was performed by the iGEM2012 team of Wageningen UR, strongly suggesting that this part can be expressed in ''E. coli'' as well. | + | The PLRV major Coat Protein should be capable of forming a Virus-Like Particle, but this was not (yet) confirmed for this part. |
| + | This gene was isolated from potato leaf roll (PLRV) infected plants. The obtained sequence of the coding gene is 100% identical to several PLRV isolates available in the NCBI database. Expression of a similar viral coat proteins was performed by the iGEM2012 team of Wageningen UR, strongly suggesting that this part can be expressed in ''E. coli'' as well. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | |||
<!-- --> | <!-- --> | ||
Revision as of 14:14, 26 September 2012
PLRV Coat Protein gene - Isolated from Bildstar potato
This part encodes the major coat protein of the Potato Leafroll Virus (PLRV). It was isolated from a PLRV infected Bildstar potato leaf by iGEM team Wageningen UR 2012. This was done by cDNA synthesis followed by PCR.
The PLRV major Coat Protein should be capable of forming a Virus-Like Particle, but this was not (yet) confirmed for this part. This gene was isolated from potato leaf roll (PLRV) infected plants. The obtained sequence of the coding gene is 100% identical to several PLRV isolates available in the NCBI database. Expression of a similar viral coat proteins was performed by the iGEM2012 team of Wageningen UR, strongly suggesting that this part can be expressed in E. coli as well.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 580
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
*Safety notice*
This part is isolated from a virus and, when assembled, will form particles that very much resemble this virus in size and shape. However, no additional viral information is stored in this part and viral infection and/or replication can therefore be ruled out. It is completely safe for use in normal laboratory circumstances.