Difference between revisions of "Part:pSB1AT3"
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pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). | pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Tetracycline resistant up to 150 ug/ml. | Tetracycline resistant up to 150 ug/ml. | ||
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<partinfo>pSB1AT3 SequenceAndFeatures</partinfo> | <partinfo>pSB1AT3 SequenceAndFeatures</partinfo> | ||
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+ | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>pSB1AT3 parameters</partinfo> | <partinfo>pSB1AT3 parameters</partinfo> | ||
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Revision as of 02:17, 5 June 2006
High copy BioBrick assembly plasmid
pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
Usage and Biology
Tetracycline resistant up to 150 ug/ml.
Kanamycin sensitive at 5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3425
Illegal NheI site found at 1270
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3431 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3425
Illegal BamHI site found at 1416
Illegal XhoI site found at 1035
Illegal XhoI site found at 2318 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3425
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3425
Plasmid lacks a suffix.
Illegal XbaI site found at 3440
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1442
Illegal NgoMIV site found at 1810
Illegal NgoMIV site found at 1970 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2464