Difference between revisions of "Part:BBa K896001:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
1. endogenious EcoRI site are
+
1. Endogenious EcoRI site is at 656bp, and PstI is at 165bp
 +
2. Instead of mutant ctting site, we create a smart pSB1C3 plasmid (MfeI-XbaI -pSB1C3-SbfI-SpeI)for CysI cloning.
 +
3. Taking advantage of MfeI and EcoRI are compatible; SbfI and XbaI are compatible.
  
 
===Source===
 
===Source===

Revision as of 14:05, 26 September 2012

CysI (Sulfite reductase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 121
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 121
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 655
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 121
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 121
    Illegal NgoMIV site found at 394
    Illegal NgoMIV site found at 580
    Illegal NgoMIV site found at 722
    Illegal AgeI site found at 94
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 130
    Illegal BsaI site found at 1452


Design Notes

1. Endogenious EcoRI site is at 656bp, and PstI is at 165bp 2. Instead of mutant ctting site, we create a smart pSB1C3 plasmid (MfeI-XbaI -pSB1C3-SbfI-SpeI)for CysI cloning. 3. Taking advantage of MfeI and EcoRI are compatible; SbfI and XbaI are compatible.

Source

YES

References

YES