Difference between revisions of "Part:BBa K733004"
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==Reference==
 
==Reference==
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Pellegrini O, Mathy N, Gogos A, Shapiro L, and Condon C. "The ''Bacillus subtilis'' ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.." ''Molecular microbiology''. 56.5 (2005): 1139-1148. Print.
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Pellegrini O, Mathy N, Gogos A, Shapiro L, and Condon C. "The ''Bacillus subtilis'' ''ydcDE'' operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.." ''Molecular microbiology''. 56.5 (2005): 1139-1148. Print.

Revision as of 14:05, 26 September 2012

RBS+ydcE

ydcE which also is named as ndoA, encoding an RNase, namely EndoA. Being in the same RNase family with MazF/ChpAK/PemK, YdcE - the endoribonuclease ydcE encodes - can inactivate cellular mRNAs by cleaving them at specific but frequently occurring sites (i.e. UAUAAU↓AC). Thus, overexpression of EndoA can lead to programmed cell death or unhealthy conditions, depending on the amount of EndoA. Note that ydcE(ndoA) is demonstrated functional both in E. coli and B. subtilis. (Pellegrini et al., 2005)

YdcE.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Pellegrini O, Mathy N, Gogos A, Shapiro L, and Condon C. "The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.." Molecular microbiology. 56.5 (2005): 1139-1148. Print.