Difference between revisions of "Part:BBa K823044"
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[[Image:SuicideSwitchgrafik.jpg|600px]] | [[Image:SuicideSwitchgrafik.jpg|600px]] | ||
− | Our team used this part in the combination with [https://parts.igem.org/ | + | Our team used this part in the combination with [https://parts.igem.org/Part:BBa_K823041 P<sub>''ydfG''</sub>],[https://parts.igem.org/Part:BBa_K823043 ecf41<sub>Bli aa 1-204</sub>],[https://parts.igem.org/wiki/index.php?title=Part:BBa_K823048 P<sub>''spoIVB''</sub>]/[https://parts.igem.org/wiki/index.php?title=Part:BBa_K823042 P<sub>''sspK''</sub>] in the [http://2012.igem.org/Team:LMU-Munich/Germination_Stop#Suicide_switch <b>Suicide</b> switch]. |
− | + | σ G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination. | |
Revision as of 14:05, 26 September 2012
MazF a bacterial toxin
MazF is a bacterial toxin from E. coli and degrades mRNA
Our team used this part in the combination with PydfG,ecf41Bli aa 1-204,PspoIVB/PsspK in the [http://2012.igem.org/Team:LMU-Munich/Germination_Stop#Suicide_switch Suicide switch].
σ G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]