Difference between revisions of "Part:BBa K733002:Design"

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===Design Notes===
 
===Design Notes===
  
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In the xylR region, there are three illegal cutting sites – one EcoRI cutting site and two XbaI cutting sites. We first check with the codon usage in ''B. subtilis'' and design a sequence for xylR without these illegal cutting sites. Then, we used PCR mutagenesis to eliminate these three illegal cutting sites.
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In the <i>xylR</i> region, there are three illegal cutting sites – one EcoRI cutting site and two XbaI cutting sites. We first check with the codon usage in ''B. subtilis'' and design a sequence for xylR without these illegal cutting sites. Then, we used PCR mutagenesis to eliminate these three illegal cutting sites.
  
 
===Source===
 
===Source===

Latest revision as of 13:55, 26 September 2012

xylR+PxylA: A xylose inducible promoter with its transcriptional regulator.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the xylR region, there are three illegal cutting sites – one EcoRI cutting site and two XbaI cutting sites. We first check with the codon usage in B. subtilis and design a sequence for xylR without these illegal cutting sites. Then, we used PCR mutagenesis to eliminate these three illegal cutting sites.

Source

We obtain this part from a plasmid named pAX01, which is from BGSC. （Zeigler 2002）

References

Zeigler, D. (2002). Integration Vectors for Gram-Positive Bacteria (7 ed.). Columbus: The Bacillus Genetic Stock Center.