Difference between revisions of "Part:BBa K934025:Experience"

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===Applications of BBa_K934025===
 
===Applications of BBa_K934025===
  
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[[Image:Pluxtet_assay.png|thumb|center|500px|]]
 
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To characterize the function of the Lux/Tet hybrid promoter, we constructed a part Plux/tet-GFP (BBa_ K934024) by inserting Plux/tet promoter in front of a GFP coding sequence. By using the reporter cell that contains Plux/tet-GFP and constitutive LuxR and TetR generator (PlacIq-LuxR-TetR), we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, 3OC6HSL and aTc (anhydrous tetracycline).
 
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In the presence of both inducers, the culture showed about 500-fold higher fluorescence intensity than that in the absence of both inducers.
 
  
 
===User Reviews===
 
===User Reviews===

Revision as of 13:42, 26 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K934025

User Reviews

UNIQbe3ef53dd0010d47-partinfo-00000000-QINU UNIQbe3ef53dd0010d47-partinfo-00000001-QINU