Difference between revisions of "Part:BBa K091134:Experience"
Nongchaoer (Talk | contribs) (→User Reviews) |
Nongchaoer (Talk | contribs) (→User Reviews) |
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When we first tested this part, it didn't work at all. Then we sequenced the part and found many problems. The Plax promoter and PlasR sequence are correct but the rest part is totally wrong. | When we first tested this part, it didn't work at all. Then we sequenced the part and found many problems. The Plax promoter and PlasR sequence are correct but the rest part is totally wrong. | ||
| − | We rebuild and improve this part by ourselves. We | + | We rebuild and improve this part by ourselves. We change GFP to RFP in this part. This is because that this RFP protein is much more easily to detect than the GFP. You don't need fluorescent microscope. Besides, we change some the sequence error and make it work finally. |
Revision as of 13:30, 26 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K091134
This part was tested by iGEM 2012 team Tsinghua.
This part can be uesd to sense the signal of C12HSL. There are many kinds of AHL molecules in nature. Their structures are similar and they share almost the same mechanism. Two AHL molecules can bind with two receptors and form a dimer of dimer. This complex can specificly activate the promoter. Different AHL molecules are from different species.
User Reviews
UNIQ3aea25c6532c3b18-partinfo-00000000-QINU UNIQ3aea25c6532c3b18-partinfo-00000001-QINU This part was tested by iGEM 2012 team Tsinghua.
When we first tested this part, it didn't work at all. Then we sequenced the part and found many problems. The Plax promoter and PlasR sequence are correct but the rest part is totally wrong.
We rebuild and improve this part by ourselves. We change GFP to RFP in this part. This is because that this RFP protein is much more easily to detect than the GFP. You don't need fluorescent microscope. Besides, we change some the sequence error and make it work finally.