Difference between revisions of "Part:BBa K091134:Experience"

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(User Reviews)
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This part was tested by iGEM 2012 team Tsinghua.
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When we first tested this part, it didn't work at all. Then we sequenced the part and found many problems. The Plax promoter and PlasR sequence are correct but the rest part is totally wrong.
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We rebuild and improve this part by ourselves. We

Revision as of 13:27, 26 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K091134

This part was tested by iGEM 2012 team Tsinghua.

This part can be uesd to sense the signal of C12HSL. There are many kinds of AHL molecules in nature. Their structures are similar and they share almost the same mechanism. Two AHL molecules can bind with two receptors and form a dimer of dimer. This complex can specificly activate the promoter. Different AHL molecules are from different species.

User Reviews

UNIQa48fee31c97fd73e-partinfo-00000000-QINU UNIQa48fee31c97fd73e-partinfo-00000001-QINU This part was tested by iGEM 2012 team Tsinghua.

When we first tested this part, it didn't work at all. Then we sequenced the part and found many problems. The Plax promoter and PlasR sequence are correct but the rest part is totally wrong.

We rebuild and improve this part by ourselves. We