Difference between revisions of "Part:BBa K784038"
 
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This part includes T7*(K1F) RNA Polymerase gene. This is orthogonal polymerases, based on T7 wt RNAP, that recognizes specifically a suitable promoter- pK1F. The T7*(K1F) RNA Polymerase was donated us by Chris Voight lab (see source).
 
This part includes T7*(K1F) RNA Polymerase gene. This is orthogonal polymerases, based on T7 wt RNAP, that recognizes specifically a suitable promoter- pK1F. The T7*(K1F) RNA Polymerase was donated us by Chris Voight lab (see source).
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The T7*(K1F) RNA Polymerase is less toxic to host cells, than the T7 wt. In order to achieve reduced levels of toxicity, Chris and his team had added N-terminal degradation tag to the T7 wt polymerase to reduce its concentration in host E.coli cell and controlled the RNAP expression using a weak ribosome-binding site and ATG start codon.
+
The T7*(K1F) RNA Polymerase is less toxic to host cells, than the T7 wt. In order to achieve reduced levels of toxicity, Chris and his team had added N-terminal degradation tag to the T7 wt polymerase to reduce its concentration in host E.coli cell and controlled the RNAP expression using a weak ribosome-binding site and ATG start codon. The specificity of the T7*(K1F) to pK1F was achieved by engineering a DNA binding domain within the RNA polymerase.  
  
 
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Latest revision as of 12:23, 26 September 2012

T7*(K1F) RNA polymerase

This part includes T7*(K1F) RNA Polymerase gene. This is orthogonal polymerases, based on T7 wt RNAP, that recognizes specifically a suitable promoter- pK1F. The T7*(K1F) RNA Polymerase was donated us by Chris Voight lab (see source). The T7*(K1F) RNA Polymerase is less toxic to host cells, than the T7 wt. In order to achieve reduced levels of toxicity, Chris and his team had added N-terminal degradation tag to the T7 wt polymerase to reduce its concentration in host E.coli cell and controlled the RNAP expression using a weak ribosome-binding site and ATG start codon. The specificity of the T7*(K1F) to pK1F was achieved by engineering a DNA binding domain within the RNA polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]