Difference between revisions of "Part:BBa K592009:Experience"

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<I>iGEM Groningen 2012</I>
 
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iGEM team Groningen has managed to couple this biobrick part with <i>B. subtilis</i> promoters: alsT, fnr, and sboA. The cloning was done in BBa_K818000 (plasmid backbone for <i>B. subtilis</i>, engineered by team Groningen 2012), to allow color expression in <i>B. subtilis</i>.
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<I>iGEM Groningen 2012</I>
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Our team has managed to couple this biobrick part with our promoters: alsT, fnr, and sboA. The cloning was done in BBa_K818000 (plasmid backbone for B. subtilis, engineered by team Groningen 2012), to allow color expression in B. subtilis. We utilized a strong RBS BBa_B0034 for pigment expression in E. coli and B. subtilis.
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The purple/blue colour was strongly visible in E.coli without any induction, while the expression in B. subtilis was more subtle (B. subtilis colony looks slightly blue on the plate agar).
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Revision as of 11:58, 26 September 2012

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Applications of BBa_K592009

User Reviews

UNIQ18e176a8647e1a1d-partinfo-00000000-QINU UNIQ18e176a8647e1a1d-partinfo-00000001-QINU

BBa_K592009 iGEM Groningen 2012

Our team has managed to couple this biobrick part with our promoters: alsT, fnr, and sboA. The cloning was done in BBa_K818000 (plasmid backbone for B. subtilis, engineered by team Groningen 2012), to allow color expression in B. subtilis. We utilized a strong RBS BBa_B0034 for pigment expression in E. coli and B. subtilis.

The purple/blue colour was strongly visible in E.coli without any induction, while the expression in B. subtilis was more subtle (B. subtilis colony looks slightly blue on the plate agar).

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