Difference between revisions of "Part:BBa K801066"
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<partinfo>BBa_K801066 short</partinfo>
 
<partinfo>BBa_K801066 short</partinfo>
  
−
This part is second version of BBa_K801060. BBa_K801060 has a C-terminal Strep-tag, which is missing here.
+
This part is second version of BBa_K801060. It contains (+)-limonene synthase and the consensus sequence for enhanced expression in yeast. It does not contain a C-terminal Strep tag as BBa_K801060. The sequence does not contain a stop codon so that RFC 25 has to be used.
 +
 
  
 
'''Does not contain stop codon. Needs to be used with RFC 25.'''
 
'''Does not contain stop codon. Needs to be used with RFC 25.'''

Revision as of 11:52, 26 September 2012

(+)-limonene synthase 1 with yeast consensus sequence for improved expression

This part is second version of BBa_K801060. It contains (+)-limonene synthase and the consensus sequence for enhanced expression in yeast. It does not contain a C-terminal Strep tag as BBa_K801060. The sequence does not contain a stop codon so that RFC 25 has to be used.


Does not contain stop codon. Needs to be used with RFC 25.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 107