Difference between revisions of "Part:BBa K782085"
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==Introduction==
 
==Introduction==
  
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Our construct contain [https://parts.igem.org/Part:BBa_K782070 TALA binding sites] that are cloned upstream of minimal promoter. Downstream of CMV is blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
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Our construct contain [https://parts.igem.org/Part:BBa_K782070 TALA binding sites] that are cloned upstream of [https://parts.igem.org/Part:BBa_K782087 minimal promoter]. Downstream of promoter [https://parts.igem.org/Part:BBa_K782065 TALA:VP16] and blue fluorescent protein.  
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BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
  
 
[[Image:BFP.png‎]]
 
[[Image:BFP.png‎]]

Revision as of 11:20, 26 September 2012

10x[TALA] operator_minimal promoter_TALA:NLS:VP16_t2a_BFP

TALA labels represents TAL effector 1257 from zebrafish experiments (Sander et al., 2011)

Introduction

Our construct contain TALA binding sites that are cloned upstream of minimal promoter. Downstream of promoter TALA:VP16 and blue fluorescent protein.


BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.

BFP.png

Figure 1: Schematic representation of the construct.


Characterization

Results: Specific TAL binding sites were further characterized with other reporter constructs.

BFP was obtained from Evrogen.

References

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 750
    Illegal BamHI site found at 720
    Illegal BamHI site found at 3262
    Illegal XhoI site found at 788
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 147
    Illegal NgoMIV site found at 507
    Illegal AgeI site found at 12
    Illegal AgeI site found at 347
    Illegal AgeI site found at 372
    Illegal AgeI site found at 707
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4207