Difference between revisions of "Part:BBa K411003:Experience"

(→‎Applications of BBa_K411003)
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ZJU-China 2012 tests K411003
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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[[Image:Riboscaffold_fig_11.jpg]]
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how you used this part and how it worked out.
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The 8 different concentration of theophylline comparision on part K411003 theophylline  robswitch tagged with GFP. Excitation at 480nm and emission at 535nm. Up to 10mM theophylline, cells didn’t show obvious side effects and GFP production is proportioned with theophylline concentration, showing that K411003 is an effective riboswitch which can be regulated by theophylline. When theophylline concentration is beyond a certain degree (about 10 mM), it somewhat affect cell growth and GFP production.
  
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[http://www.baidu.com try]
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Results show that this is an effecitve riboswitch regulated by theophylline.
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===User Reviews===
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<!-- DON'T DELETE --><partinfo>BBa_K411003 StartReviews</partinfo>
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<!-- Template for a user review
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{|width='80%' style='border:1px solid gray'
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<partinfo>BBa_K411003 AddReview number</partinfo>
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<I>Username</I>
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Enter the review inofrmation here.
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<partinfo>BBa_K411001 AddReview number</partinfo>
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<I>NYMU-Taipei iGEM10</I>
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[http://2010.igem.org/Team:NYMU-Taipei/Experiments/Speedy_switch Experimental Data] by NYMU-Taipei iGEM10 using this part to test [[Part:BBa_K411001]]. Works. Downstream translation strength is proportional to the amount of theophylline added.
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|};
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<!-- DON'T DELETE --><partinfo>BBa_K411003 EndReviews</partinfo>
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Revision as of 11:09, 26 September 2012

ZJU-China 2012 tests K411003 Riboscaffold fig 11.jpg The 8 different concentration of theophylline comparision on part K411003 theophylline robswitch tagged with GFP. Excitation at 480nm and emission at 535nm. Up to 10mM theophylline, cells didn’t show obvious side effects and GFP production is proportioned with theophylline concentration, showing that K411003 is an effective riboswitch which can be regulated by theophylline. When theophylline concentration is beyond a certain degree (about 10 mM), it somewhat affect cell growth and GFP production.

Results show that this is an effecitve riboswitch regulated by theophylline.